Ĭurrently, the submerged fermentation of Ganoderma is viewed as a promising approach for the efficient production of these polysaccharides and triterpenes because it is fast, cost-effective, and it is easy to control the product quality. Recent studies have revealed that Ganoderma TA has a few important pharmacological activities that include antitumor effects, inhibition of cholesterol synthesis, anti-HIV-1 and anti-HIV-protease activities, and inhibition of U46619-induced platelet aggregation. Two biologically active compounds that have been demonstrated to possess diverse and potentially significant pharmacological activities are polysaccharides and triterpenes (especially triterpene acids, TA). Modern chemistry has demonstrated that Ganoderma species contain a variety of phytochemicals. lucidum has a white pore surface and thin dissepiments (40–80 μm)). lucidum are the presence of 1–2 black melanoid bands in the context, a yellow pore surface and thick dissepiments (80–120 μm) at maturity (in contrast, G. The most striking characteristics that differentiate G. Dai, which is distributed in East Asia, was proposed. ex Fr.) Karst was originally described as coming from Europe and is now known in East Asia, Europe and North America. The medicinal values of this mushroom were documented thousands of years ago. Ganoderma (Ganodermaceae) is a genus of edible and medicinal mushroom that is known as “reishi” in Japanese and “lingzhi” in Chinese. Chemical analysis revealed that the key active triterpene compounds, ganoderic acid T and ganoderic acid Me, predominated in the extract. lingzhi mycelia was found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro, and the TAE exhibited dose-dependent antitumor activity against the solid tumor sarcoma 180 in vivo. Additionally, the triterpene acid extract (TAE) from G. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and maximum TA production and productivity of 295.3 mg/L and 49.2 mg/L/day were achieved, which represented 80.9% and 111.5% increases, respectively, compared with the non-optimized conditions. These conditions resulted in a TA yield of 308.1 mg/L in a 5-L stirred bioreactor. The optimum conditions were an initial pH of 5.9, 20.0% DO and 28.6 ☌. The interactions of three variables were studied using a Box-Benhnken design, namely initial pH, dissolved oxygen (DO) and fermentation temperature. lingzhi using response surface methodology. In this study, enhanced TA yield and productivity were attained with G. lingzhi submerged culture mycelia possess antitumor activity needs to be further proven. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi.
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